Engineering genetically encoded biosensors of neural activity and metabolism

Advances in microscopy and fluorescent probe development are revolutionizing biological research by enabling the normally achromatic world of the cell to be visualized in high resolution and with vivid colours. A major focus of the Campbell research group is the use of protein engineering for the development of fluorescent protein-based biosensors for imaging of cell signalling and metabolism. Protein engineering, using a combination of rational protein design and directed protein evolution, is the most effective and versatile approach for generating such biosensors. Accordingly, by exploiting structure-guided design, combined with iterative cycles of high-throughput fluorescence image-based screening of bacterial colonies, and lower throughput testing of promising variants in mammalian cells, we are developing a growing selection of fluorescent protein-based biosensors with improved properties. In this seminar I will present some of our most recent efforts to expand the palette of calcium ion biosensors, and describe how we are using similar engineering efforts to make biosensors for biologically-active ions and key metabolites.

Host: Dr. Stephen Withers