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UBC Tissue Clearing and Expansion 2021

September 22, 2021 - 10:00 am - September 23, 2021 - 1:00 pm

BCREGMED and the Dynamic Brain Circuits in Health & Disease team are excited to offer a TWO DAY online workshop to facilitate the use of tissue clearing and expansion techniques in the research lab.

September 22nd and 23rd
10AM – 1PM both days
ZOOM

Purpose
Biological specimens are intrinsically difficult to image.  Currently used tools and methods, which cut two-dimensional sections of tissue samples to provide insight into the cellular components, lack in their ability to reveal the systemic nature of tissues and organs which are inherently three-dimensional. On the other hand, imaging deep into intact tissues brings with it its own problems as a result of the obscuring effects of light scatter and the limitations of most commercial microscopes.

Techniques to eliminate this scatter and provide understanding into the true behavior of tissues and organs have been developed, however to our knowledge these techniques being under-utilized in research labs in BC due to their complexity and lack of appropriate equipment. With the recent purchase of a light sheet microscope for the School of Biomedical Engineering and a lattice light sheet microscope expected at the Centre for Brain Health, tissue clearing and expansion will be made possible however training is needed on the various protocols of expansion and clearing, 3D image analysis, and troubleshooting to make sure these techniques are implemented effectively.

Details

Start:
September 22, 2021 - 10:00 am
End:
September 23, 2021 - 1:00 pm
Website:
https://bcregmed.ca/event/tissue-clearing-and-expansion-workshop-2-0/2021-09-22/

Venue

Online

Organizer

BCREGMED