Micropatterning technology to spatially guide the self-assembly of human pluripotent stem cells (hPSCs) into neural tissues has advanced our ability to model early stages of human brain development. Current approaches for hPSC micropatterning rely on specialized materials that limit accessibility and throughput. To address these limitations, we developed a simple and scalable bioprinting-based approach to generate micropatterned, scaffolded neuroepithelial tissues with a single lumen in 6 days.
